| CSAD

The enzyme sulfinoalanine decarboxylase catalyzes the chemical reaction 3-sulfino-L-alanine ⇌ {\displaystyle \rightleftharpoons } hypotaurine + CO2 Hence, this enzyme has one substrate, 3-sulfino-L-alanine (also known as Cysteine sulfinic acid), and two products, hypotaurine and CO2.This enzyme belongs to the family of lyases, specifically the carboxy-lyases, which cleave carbon-carbon bonds. The systematic name of this enzyme class is 3-sulfino-L-alanine carboxy-lyase (hypotaurine-forming).
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.27	2.06	1.65	1.15	0.72	0.45	0.29	0.05
Corrected OD	2.22	2.01	1.6	1.1	0.67	0.4	0.24	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human CSAD spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	86-101	96
Cell Culture Supernates(n=8)	92-105	103

 

| Linearity

Samples were spiked with high concentrations of Human CSAD and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-98
	Average (%)	91	93
1 : 4	Range (%)	89-103	87-108
	Average (%)	95	98